Charge determination of membrane molecules in polymer-supported lipid layers
نویسندگان
چکیده
منابع مشابه
Polymer-Induced Swelling of Solid-Supported Lipid Membranes.
In this paper, we study the interaction of charged polymers with solid-supported 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) membranes by in-situ neutron reflectivity. We observe an enormous swelling of the oligolamellar lipid bilayer stacks after incubation in solutions of poly(allylamine hydrochloride) (PAH) in D₂O. The positively charged polyelectrolyte molecules interact with the lip...
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Much of the short-range forces and structures of softly supported DMPC bilayers has been described previously. However, one interesting feature of the measured force-distance profile that remained unexplained is the presence of a long-range exponentially decaying repulsive force that is not observed between rigidly supported bilayers on solid mica substrate surfaces. This observation is discuss...
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Solid supported bilayer lipid membranes are model systems to mimic natural cell membranes in order to understand structural and functional properties of such systems. The use of a model system allows for the use of a wide variety of analytical tools including atomic force microscopy, impedance spectroscopy, neutron reflectometry, and surface plasmon resonance spectroscopy. Among the large numbe...
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Solid-supported lipid membranes are important for their roles in fundamental biophysical research as well as in applications such as biosensors. In our study, lipopolymers containing alkyl side chains were synthesized and a mixture of the lipopolymer and free lipids was preorganized at the air-water interface and then transferred to a solid substrate using the Langmuir-Blodgett technique. A pho...
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This work examines polyelectrolyte adsorption (exclusively driven by electrostatic attractions) for a model system (DMAEMA, polydimethylaminoethyl methacrylate, adsorbing onto silica) where the adsorbing polycation is more densely charged than the substrate. Variations in the relative charge densities of the polymer and substrate are accomplished by pH, and the polycation is of sufficiently low...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Biomembranes
سال: 1995
ISSN: 0005-2736
DOI: 10.1016/0005-2736(95)00129-q